
01-870-1A ·
Sartorius · Cat: 01-870-1A
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shop.sartorius.com/in/p/tae-electrophoresis-buffer-x50-500ml...Description
TAE Buffer 50X is a solution used in Agarose Gel Electrophoresis AGE typically for the separation of nucleic acids i.e. DNA and RNA and as a running buffer for preparative work.Tris-Acetate-EDTA TAE is not only used in nucleic acid agarose and polyacrylamide gel electrophoresis but also in agarose and polyacrylamide gel preparation. Instructions for use Take a bottle of TAE Electrophoresis Buffer 50X from specified storage conditions at 15-30C and read the label. Ensure that the cap of the bottle is tight. Gently swirl the solution in the bottle to ensure homogeneity. Wipe the outside of the bottle with a disinfectant solution such as 70 % ethanol. Using aseptic/sterile technique under a laminar-flow culture hood and work according to established protocols. The TAE Electrophoresis Buffer Concentrate should be diluted to a working concentration of 1X before use. For each Electrophoresis, use fresh TAE Buffer at the working concentration of 1X