TaKaRa Z-TaqTM DNA Polymerase

Description

Takara Z-Taqis characterized by 5-fold greater processivity thanTaqDNA Polymerase, resulting in rapid amplification times in PCR applications. Separate tubs of optimzed buffer Mg2+plus , and dNTP mix are supplied with the polymerase. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Amplification of long products using TakaraZ-TaqDNA Polymerase. Long products 7.5 kb, 17.5 kb, 18 kb, or 20 kb were amplified from human genomic DNA 500 ng/50 mul reaction orE. coligenomic DNA 100 ng/50 mul reaction using the following conditions: Human 7.5 kb product 30 cycles of: 98C, 5 sec.; 68C, 80 sec. E. coli18 and 20 kb products and human 17.5 kb product 35 cycles of: 98C, 5 sec.; 68C, 180 sec. All reactions were performed using a TaKaRa PCR Thermal Cycler PERSONAL discontinued using the FAST cycling mode. After PCR, 5 mul of the reaction mixture was loaded on an agarose gel for electrophoresis. Takara BiosZ-TaqDNA Polymerase efficiently amplified large genomic fragments, including a 20 kb target. Back