TB Green Advantage qPCR Premix

Description

TB Green Advantage qPCR Premix is a convenient 2X master mix containing TB Green dye, full-lengthTaqDNA Polymerase, hot-start antibody, dNTPs, and buffer including Mg2+ . The mix comes with two separate tubes of ROX passive reference dye: ROX Reference Dye LSR contains the optimal concentration of ROX for instruments whose excitation source is a 488 nm laser; and ROX Reference Dye LMP contains the optimal concentration of ROX for instruments whose excitation source is either a lamp or an LED. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Reaction specificityperformance of Takara Bio TB Green Advantage qPCR Premix vs. Competitor Rs mix using a Roche LightCycler.The results for the Takara Bio reagent are shown in Panels A and C, and those for Competitor Rs reagent are shown in Panels B and D. The cycling conditions for our reagent consisted of 1 cycle at 95C for 10 min, followed by 45 cycles at 95C for 5 sec and 60C for 20 sec. For Competitor Rs reagent, the cycling conditions consisted of 1 cycle at 95C for 10 min, followed by 45 cycles at 94C for 10 sec, 55C for 5 sec, and 72C for 10 sec. Back Amplification efficiencyperformance of TB Green Advantage qPCR Premix vs. Competitor As SYBR mix using an ABI PRISM 7000 Sequence Detection System.The results for the TB Green reagent are shown in Panels A and C, and those for Competitor As reagent are shown in Panels B and D. The cycling conditions for the TB Green reagent consisted of 1 cycle at 95C for 10 sec, followed by 40 cycles at 95C for 5 sec and 60C for 31 sec. For Competitor As reagent, the cycling conditions consisted of 1 cycle at 95C for 10 min, followed by 45 cycles at 95C for 15 sec and 60C for 1 min. Back Reaction specificityperformance of Takara Bios TB Green Advantage qPCR Premix vs. Competitor Is qPCR mix using a Cepheid Smart Cycler.The results for the Takara Bio reagent are shown in Panels A and C, and those for Competitor Is reagent are shown in Panels B and D. The cycling conditions for the TB Green reagent consisted of 1 cycle at 95C for 2 min, followed by 45 cycles at 95C for 5 sec and 60C for 20 sec. For Competitor Is reagent, the cycling conditions consisted of 1 cycle at 95C for 2 min, followed by 45 cycles at 95C for 15 sec and 60C for 30 sec. Back