THIOGEL MEDIUM

Description

Proteolytic organisms digest proteins and consequently liquefy gelatin or coagulated serum. Liquefaction of gelatin, being the commonest proteolytic property, is routinely used as an index of proteolytic activity. Gelatin will not by itself support the growth of many pathogens and is therefore incorporated into a nutrient medium. In Thiogel Medium, gelatin is incorporated into Thioglycollate Medium without Indicator. Thioglycollate Medium was modified by Brewer by replacing meat infusion in original formulation by plant soya and casein peptones to enhance growth. Thioglycollate Medium is used for cultivation of strict anaerobes, microaerophiles and aerobic microorganisms and for identifying the pure cultures on the basis of their ability to liquefy gelatin.