TLR9 Knockout Immortalized Mouse Dendritic Cell Line

Description

As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC cDC acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The MutuDCTlr9-/- cell line is an immortalized splenic CD8alpha+ subset CD11chigh,B220-,DEC205+,CD24high,CD11b- of conventional dendritic cells derived from the TLR9 knockout CD11c:SV40LgT transgenic mice. The MutuDCTlr9-/- cell line retains response to TLR ligands such as PolyIC TLR3-L and to a lesser extent LPS TLR4-L but not CpG TLR9-L . Like the Wild-type MutuDC cells Cat. No. T0528 , these cells are capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens. Together with TLR3 knockout Cat. No. T3034 and Ifnar1 knockout Cat. No. T3036 MutuDC, these dendritic cell lines provide a powerful tool in vaccine science and immunotherapy, particularly in strategizing target antigens to CD8alpha+ subset.