TO1-3PEG-Biotin Fluorophore

Description

RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange TO bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer KD 3nM , and the strong 1000X enhancement of the dyes fluorescence when bound to the Mango apatmer Fluorescent enhancement FE=1,100 .The TO dye has a number of other desirable properties including: small size lack of toxicity plasma and nuclear membrane permeability short intracellular half-life the accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure TO1-biotin is the standard variety of TO dye for RNA Mango experiments; other dye variants are available. Learn more about the RNA Mango technology here. Watch RNA Mango in Action Transcription reaction were carried out in 300 muL volumes using T7 RNA polymerase 400 U, 50U/muL, applied biological materials , 0.5 muM TO1-3PEG-Biotin applied biological materials , in 8 mM GTP, 5 mM CTP and ATP, 2 mM UTP, 40 mM TRIS buffer pH 7.9, 2.5 mM spermidine, 26 mM MgCl2, 20 mM KCl, Pyrophosphatase 0.5 U, 0.1 U/muL, ThermoFisher Scientific , and 0.01 % Triton X-100. To each sample, either water Negative , 0.33 muM DNA template Mango Transcription , or 500 nM final Mango III A10U RNA Positive was added. Samples were visualized in a blue light box, movie is played back at 30X speed. The most advanced RNA tracking, visualization and pull down technology. Technology for studying the diverse cellular roles of RNA has lagged behind the tools for studying DNA and proteins, but innovative researchers are working to change that One such researcher is Dr. Peter Unrau of Simon Fraser University. He and his team have created RNA Mango, a novel technology with a number of useful applications.