(+/-)-trans-1,2-Bis(2-mercaptoacetamido)cyclohexane

Description

AAasAasAA+/- -trans-1,2-Bis 2-mercaptoacetamido cyclohexane is a small-molecule dithiol catalyst with a low thiol pKa value 8.3 and high reduction potential -0.24 V , capable of mimicking PDI activity. It catalyzes the activation of scrambled ribonuclease A scrambled ribonuclease A and promotes the formation of native disulfide bonds, thereby significantly enhancing protein folding efficiency. Adding AAasAasAA+/- -trans-1,2-Bis 2-mercaptoacetamido cyclohexane to the culture medium of Saccharomyces cerevisiae can increase the secretion of exogenously expressed Schizosaccharomyces pombe acid phosphatase by more than threefold. AAasAasAA+/- -trans-1,2-Bis 2-mercaptoacetamido cyclohexane holds great potential for applications in protein production and secretion research.