Tris-EDTA Antigen Retrieval Buffer (10×, pH 9.0)

Description

After fixation with paraformaldehyde, formalin or other aldehyde-based reagents, antigenic determinants become masked by aldehyde cross-links, carboxymethylation, and protein–protein cross-linking. These modifications alter protein conformation, reduce the number of accessible epitopes, and lead to false-negative results or weak staining in immunolabeling. The aldehyde-induced cross-links are reversible; under appropriate conditions (e.g., high temperature or enzymatic digestion) the original protein conformation can be restored. This process is termed antigen retrieval (AR) and is a critical step in immunohistochemistry. Multiple retrieval methods are available; heat-induced epitope retrieval (HIER) or enzymatic retrieval can be selected according to tissue type and antigen characteristics.This product, Tris-EDTA Antigen Retrieval Buffer (10×, pH 9.0), is a concentrated buffer. Dilute 10-fold to obtain a working solution of pH 9.0 containing 1 mM EDTA, suitable for antigen retrieval.This production can be used with mIHC,IHC,IF