Tyramide Amplification Buffer

Description

Tyramide Amplification Buffer is a ready-to-use buffer primarily intended for immunostaining of cells and tissues by the technique of tyramide signal amplification TSA . TSA Technology is based on a tyrosinase labeling system that reacts a tyrosine-labeled antibody with a fluorescently labeled tyramide using peroxidase to form a highly amplified fluorescent signal. For example, horseradish peroxidase HRP can catalyze the reaction of tyrosine and hydrogen peroxide under milder conditions to generate epoxy groups. Epoxy groups promote the binding of tyramide to adjacent amino acids to form fluorescently labeled products.