U251MG Stably Expressing Tet-on Cell Line

Description

U251MG cells were stably transfected with the rtTA expression vector to generate the U251MG Stably Expressing Tet-on Cell Line. It can be used in vitro to stably transfect gene of interest cloned in tetracycline-responsive expression vector i.e. pTRE2 with Hygromycin selection and pick clones. With the addition of 1 mug/ml doxycycline, end users can test individually for induced gene expression under the tet-regulated promoter.