Ultra-Pure Phi29 DNA Polymerase

Description

Ultra-Pure Phi29 DNA Polymerase is a highly processive enzyme known for its powerful strand-displacement activity, making it ideal for isothermal amplification of both circular and linear DNA templates. It is particularly effective in techniques such as Rolling Circle Amplification RCA , Multiple Displacement Amplification MDA , and Whole Genome Amplification WGA . Thanks to its 35 exonuclease activity, Ultra-Pure Phi29 DNA Polymerase boasts exceptional fidelity, enabling the amplification of even very small quantities of DNA templates. The enzyme undergoes an advanced, multi-step purification process that utilizes a combination of physical, chemical, and enzymatic methods to ensure the complete removal of contaminating genomic DNA. Rigorous quality control is performed, including a non-specific DNase Activity Assay and a qPCR DNA Contamination Test using a TaqMan probe, ensuring superior purity and performance. Product Component Quantity Ultra-Pure Phi29 DNA Polymerase 100 mul 200 rxn 5X Ultra-Pure Phi29 DNA Polymerase Reaction Buffer1 500 mul 1Buffer contains 0.5 mug/mul BSA. Keep at -20C at all times except when using the product.