UREA AGAR BASE (CHRISTENSEN) (AUTOCLAVABLE) (VEG.)

Description

Urea Veg Agar Base is prepared by completely replacing animal based peptones with vegetable peptones which makes the medium free of BSE/TSE risks. Urea Veg Agar Base is the modification of Urea Agar Base formulated in accordance with Christensen formulation who modified the original medium formulated by Rustigian and Stuart. This medium differentiates between rapid urease positive Proteus species, other urease positive organisms like Citrobacter, Enterobacter, Klebsiella and bacteria other than Enterobacteriaceae. Heavy inoculum of growth is inoculated on the surface of the slants. When urea is utilized, ammonia formed during incubation makes the medium alkaline, showing a pink- red colour due to the phenol red indicator. Prolonged incubation may cause alkaline reaction in the medium. Check using medium without urea as the negative control.