V5-Tag Rapid Competitive Assay Kit

Description

V5-Tag Rapid Competitive Assay Kit adopts colloidal gold lateral flow chromatography technology and is developed based on competitive immunoassay principle. Recombinant V5-Tag protein is immobilized on the chromatographic membrane as test line, and secondary antibody for colloidal gold conjugated antibody is fixed as control line; colloidal gold-labeled anti-V5-Tag monoclonal antibody is pre-coated on the conjugate pad. If no V5-Tag protein exists in samples, gold-labeled antibodies migrate freely to the test line and bind with immobilized V5-Tag protein to form distinct purplish-red bands. When V5-Tag target protein is contained in samples, the target binds preferentially to gold-labeled antibodies and occupies antigen-binding sites, blocking the combination between gold-conjugated antibodies and immobilized protein on test line. Higher concentration of V5-Tag protein leads to lighter color of test line; full disappearance of test line occurs above 18 μg/mL protein concentration. The limit of detection is 2 μg/mL, and test line color intensity is negatively correlated with protein concentration within 2 μg/mL ~ 18 μg/mL.