Vero Cell 2G HCP ELISA Kit

Description

Expression of viral vectors and vaccines in Vero cells is a cost-effective method for their production in commercial quantities. The manufacturing and purification process of these products leaves the potential for impurities by Vero cell host cell proteins. Such impurities can reduce the efficacy of the therapeutic or vaccine agent and result in adverse toxic or immunological reactions. Thus, it is desirable to reduce HCP impurities to the lowest levels practical. This Vero Cell HCP ELISA kit was developed by generating and affinity-purifying antibodies against a mild lysate of Vero cells. This simple to use, robust, and semi-quantitative ELISA is a sensitive and specific method to aid in optimal purification process development, process control, routine quality control, and product release testing. This ELISA kit is generic in the sense that it is intended to react with a majority of Vero HCPs that could pollute the final drug product independent of the purification process. This Vero Cell HCP ELISA kit has an LOD of 1.4 ng/ml. Please use Sample Diluent, Item No. I028 , with these kits; available separately.