Von Kossa Calcium Staining Kit

Description

EnkiLife provide Von Kossa Calcium Staining Kit. Calcium is abundant in the human body, forming the skeleton as a structural support. It also plays vital roles in secretion, transport, muscle contraction, and nerve conduction. Calcium exists in two forms: Ionic calcium – found in blood circulation (i.e., blood calcium); Bound calcium – combined with proteins, carbonates, or phosphates and deposited in tissues. Apart from bones and teeth, calcium is normally present in all tissues and cells, usually not in solid form. However, under certain pathological conditions, calcium may precipitate and deposit in tissues as solid particles, a process known as pathological calcification. The deposited calcium salts are mainly calcium phosphate, followed by calcium carbonate. Calcium salts are usually mono-refringent, except for calcium oxalate, which is bi-refringent. In H&E staining, calcium generally appears purple-blue. Many dyes can form chelates with calcium, including Alizarin Red S, Purpurin, and Nuclear Fast Red. Alizarin Red S, an anthraquinone derivative and sodium salt of alizarin sulfonate, can chelate with calcium in calcium carbonate or phosphate to form an orange-red complex.Common calcium staining methods include the silver nitrate method and Alizarin Red S method. Von Kossa staining (silver method) is a metal substitution technique. When Von Kossa silver solution reacts with sections containing insoluble calcium salts, calcium is replaced by silver. Upon light exposure, the silver salt is reduced to black metallic silver, making this method suitable for high-throughput calcium staining in tissue sections.Application: Von Kossa stain