XfectTM mESC Transfection Reagent

Description

Xfect mESC is a transfection reagent that creates biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mouse embryonic stem cells. Transfections can be carried out entirely in the presence of serum. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent.ES-E14TG2a mES cells were transfected with 5 mug of DsRed-Express2 plasmid using 250 mug of Xfect mESC Transfection Reagent. 48 hr posttransfection, transfection efficiency was assessed via flow cytometry left panel as well as phase and fluorescent microscopy middle and right panels, respectively . As quantified by flow cytometric analysis, 93.9 % of the cells transfected with Xfect mESC Transfection Reagent were positive for DsRed-Express2 expression left panel , with an MFI of 716.9 data not shown . Back Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells.Xfect mESC Transfection Reagent and three competitor reagents were used according to their respective protocols to transfect mES cell lines using plasmid DNA encodingAcGFP1, in a 6-well format. 48 hr posttransfection,AcGFP1expression was assessed by flow cytometry in order to determine transfection efficiency. Back