YEAST MALT BROTH (YM BROTH) (VEG.)

Description

Yeast Malt Veg Broth are the modification of Yeast Malt Broth formulated as per Wickerham for isolation and cultivation of yeasts, moulds and other aciduric microorganisms. Fungistatic materials such as sodium propionate and diphenyl are added to YM Veg Broth to eliminate moulds and thus permits enumeration of yeasts from mixed population. Yeast Malt Veg Broth can be used as an enrichment medium for yeasts by adding a layer of sterile paraffin oil (about 1 cm) on the surface of inoculated broth. After the growth occurs it should be streaked on YM Veg Agar to obtain isolated colonies of fermentative species. To isolate fermentative as well as oxidative strains, acidified YM Veg Broth is placed on a rotary shaker for 1 or 2 days which favours development of yeast cells while the sporulation of molds is prevented. Yeasts can be further isolated by streaking on YM Agar.