YEAST MOULD CHLORAMPHENICOL AGAR, MODIFIED

Description

Yeast Mould Chloramphenicol Agar, Modified is recommended for enumeration of yeasts and moulds from foods using membrane filter technique. ISO-Grid and Neo-Grid protocols of filtration can be adopted for the same. To quantify the number of yeasts and moulds, a known amount of sample homogenate of food is prepared. Further 1 ml of homogenate is passed through the pre filter and ISO GRID membrane filter. The membrane filter is then placed on surface of Yeast Mould Chloramphenicol Agar, Modified plate. The surface of plate should be dry to avoid merging of developed colonies. Plates should be incubated in inverted position. Results may be interpreted after 48-52 hours on incubation at 20-25?C.It may be incubated further upto 72 hours for slow growing yeasts. The membrane filter is examined for developed colonies. Yeasts develop as blue coloured colonies while molds as blue-grey in colour.