YPD BROTH (YEPD BROTH)

Description

YPD Broth (YEPD Broth) is recommended by Sherman for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts. Yeasts grow well on a minimal medium containing only dextrose and salts. The addition of protein and yeast cell extract hydrolysates allows faster growth so that during exponential or log-phase growth, the cells divide every 90 minutes.